The procedures described in this protocol will allow the reader to take full advantage of the combination of two powerful techniques, lentiviral vector technology for gene transfer and gene silencing ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...

Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...