A collection of protocols covering a variety of methods to achieve super resolution in microscopy to include structured illumination, single-molecule fluorescence and localization-based approaches.

Inside living cells, mitochondria divide, lysosomes travel, and synaptic vesicles pulse—all in three dimensions (3Ds) and constant motion. Capturing these events with clarity is vital not just for ...

When single-molecule super-resolution microscopes were first commercialized some 15 years ago, they made headlines for their ability to resolve individual molecules and structures at the nanometer ...